Ethylacetate Leaf Extract of Adansonia digitata Linn Mitigates Against Redox Imbalance and Inflammatory Disorders Associated with Ageing in Liver of Male Wistar Rat
Keywords:
Adansonia digitata linn,, Antioxidant, Anti-inflammatory, Anti-ageing, D-galactoseAbstract
Ageing is driven by oxidative stress, chronic inflammation, and impaired cellular repair. However, Adansonia digitata linn is employed as an anti-ageing remedy in West Africa with limited scientific basis. Hence, this study evaluated the potential of Adansonia digitata Ethylacetate Leaf Extract (ADELE) in mitigating against redox imbalance and inflammatory disorders associated with ageing in hepatic tissue of male Wistar rats. Ethylacetate extract of the powdered leaves of the plant was prepared by cold extraction. Sixty-three (63) male Wistar rats weighing an average of 110 g were randomly allocated into 9 groups of seven (7) rats each. Group I (control), Group II (ADELE-only 200 mg/kg body weight (bw)), Group III (Vitamin E-only 200 mg/kg bw), Group IV (D-galactose-only) were treated for 8 weeks, and pretreatment groups receiving graded doses of ADELE and/or Vitamin E 4 weeks prior to D-galactose administration for an additional 4 weeks: Group V (ADELE 100 mg/kg bw and D-gal), Group VI (ADELE 200 mg/kg bw and D-gal), Group VII (ADELE 400 mg/kg bw and D-gal), Group VIII (Vit. E 200 mg/kg bw and D-gal) and Group IX (ADELE 100 mg/kg bw and Vit. E 100 mg/kg bw and D-gal). ADELE was administered orally in 0.3 ml distilled water while D-galactose was administered intraperitoneally (IP) in 0.3 ml of 0.9% normal saline daily and animals were sacrificed after eight weeks. Liver indices [Alanine transaminase (ALT), Aspartate transaminase (AST) and Gamma-glutamyl transferase (GGT) activities and total bilirubin concentrations] in serum, antioxidant indices [Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) activities, Reduced glutathione (GSH), Malondialdehyde (MDA) and total protein concentrations], and inflammatory indices [Tumour Necrosis Factor (TNF-α) and Interleukin-6 (IL-6) concentrations] in liver homogenates were evaluated using standard biochemical methods. Data were analysed using GraphPad Prism 5 at (P < 0.05) level of significance. Results showed induction of ageing by D-galactose (Group IV) with significant increase (P < 0.05) in liver indices (ALT: 84.01 ± 3.86, AST: 95.17 ± 2.33, GGT: 29.49 ± 1.70 and total bilirubin: 50.70 ± 2.87), lipid peroxidation (MDA: 10.26 ± 0.89), inflammatory cytokines (TNF-α: 6709 ± 833.00 and IL-6: 528.9 ± 35.31), and concomitant reductions in antioxidant enzyme activities (SOD: 0.69 ± 1.11, CAT: 4.07 ± 0.33 and GPx: 4.07 ± 0.13), as well as GSH: 3.60 ± 0.24 and hepatic total protein (TP): 27.04 ± 1.36, compared with controls (ALT: 70.09 ± 3.23, AST: 76.35 ± 2.51, GGT: 15.36 ± 0.52, total bilirubin: 38.74 ± 0.98, MDA: 3.84 ± 0.57, TNF-α: 1268 ± 82.66, IL-6: 345.6 ± 29.94, SOD: 1.43 ± 1.60, CAT: 5.19 ± 0.13, GPx: 6.07 ± 0.11, GSH: 4.18 ± 0.16, hepatic total protein: 38.00 ± 0.72). Pretreatment groups (V, VI, VII, VIII and IX) markedly attenuated D-galactose-induced alterations, restoring most biochemical parameters toward control levels, with some parameters showing dose-dependent activity. Histological examination revealed dose-dependent improvement in D-galactose-altered hepatic architecture toward control levels. ADELE exhibited antioxidant, anti-inflammatory, and tissue-protective potential; hence, its bioactive components may be employed in the treatment of oxidative stress- and inflammatory-related disorders as well as in the management of ageing.
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REFERENCES
Abdulwaliyu, I., Arekemase, S. O., Batari, M. L., Oshodin, J. O., Mustapha, R. A., Ibrahim, D., and Ekere, A. T. (2024). Nutritional and pharmacological attributes of baobab (Adansonia digitata) fruit pulp. Food Production, Processing and Nutrition, 6, 98.
Abuljadayel, J. A. (2023). The potential uses of baobab tree's medicinal effects in dentistry: A literature review. Cureus, 15(11), e49304. https://doi.org/10.7759/cureus.49304
Adedosu, O. T., Badmus, J. A., Adeleke, G. E., and Olalere, G. O. (2017). Thaumatococcus daniellii extract modulates glibenclamide activity and ameliorates heamatological disorders, oxidative stress and dyslipidemia associated with diabetes mellitus in rats. British Journal of Pharmaceutical Research Vol. 16, 1-12.
Adedosu, Olaniyi Temitope; Adeleke Gbadebo Emmanuel; Badmus Jelili Abiodun; Akinboro Adetayo; Adedosu Akinola Nelson; AkintolaAdebola; Babatunde Adetunji Ayodele; Ogunsina Faith Ayotunde; Oyedeji Tolulope Adejoke; Afolabi Adeniyi Stephen; Masaudat Adeola Adebayo; Emmanuel Olatunde Olagoke (2025) Methotrexate-Induced Tissue Toxicity and Cancer Herbal Management: Anti Oxidative and Protective Roles of Extract of Lannae Egregia Leaf. International Journal of Innovative Science and Research Technology, 10(8), 3303-3323. https://doi.org/10.38124/ijisrt/25aug1397.
Adegoke, A. M., Adegoke, A. M., Gbadegesin, M. A., and Odunola, O. A. (2017). Methanol extract of Adansonia digitata leaf protects against sodium arsenite-induced toxicities in male Wistar rats. Pharmacognosy Research, 9(1), 7–11. https://doi.org/10.4103/0974-8490.199761
Aebi, H., (1984).Catalase: in method of enzymatic analysis, Bergmeyer,H.U:J Bergmeyer, M. grassl, D.W.moss, H,Fritz IR.F. Massageff and M.Qellench (Eds.) 3rd Edn, verlag chemie, weinheim, Germany ISBN 13 : 9783527260430,PP: 273 – 277.
Akintola, A. O., David, K. B., Saka, W. A., Ajoke, O. E., Akinpelu, T. A., and Akinloye, M. (2021). Protective effects of Adansonia digitata leaf extract against doxorubicin-induced cardiotoxicity in albino rats. Annual Research and Review in Biology, 36(7), 12–22.
Andersen, H.R., Nielsen, J.B., Nielsen, F. and Grandjean, P. antioxidative Enzyme activities in human erythrocytes, Journal of Clinical Chemistry, 1997,43(4), pp. 562-568.
Anderson, M.E. (1985):Determination of Glutathione and Glutathione disulfide in biological samples. Methods in Enzymology, 1985,113,pp. 548-555.
Arya, Kumar Shalendra, Jyoti Kaushal, Seema Mehandia, Gursharan Singh and Arun Raina, (2018): Catalase enzyme: Application in bioremediation and food industry. Biocatalysis and Agricultural Biotechnology, Volume 18, pages 192-199.
Atuadu, V., Benneth, B.-A., Oyem, J., Esom, E., Mba, C., Nebo, K., Ezemeka, G., and Anibeze, C. (2020). Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats. Drug Metabolism and Personalized Therapy. https://doi.org/10.1515/dmpt-2020-0114
Azman, Khairunnuur Fairuz, Afifa safdar, Rahimah Zakaria (2021): D-galactose – induced liver aging model: its underlying mechanisms and potential therapeutic interventions. Journal of Experimental Gerontology volume 150, 111372.
Baker, Alison, Chi-Chuan Lin, Casey Lett, Barbara Karpinska, Megan H. Wright, Christine H. Foyer (2023): Catalase: A critical node in the regulation of cell fate Journal of Free Radical Biology and Medicine Volume 199, April, pages 56-66.
Baradaran Behzad, Farid Ghorbaninezhad, Patrizia Leone, Hajah Alemohammad, Basira Najafzadeh, Niloufar sadat Nourbakhsh, Marcella Prete, Eleonora Malerba, Hossein Saeedi, Neda Jalili Tabrizi, Vito Racanelli (2022): Tumor necrosis factor-α in systemic lupus erythematosus: Structure, function and Therapeautic implication (Review). International Journal of molecular medicine. No 43, Volume 49 issue 44. Doi.org/10.3892/ijmm.5098.
Bazile, Cassandra, Magdy M. Abdel Malik, Courtney Ackeifi, Randy L. Anderson, Roy W. beck, Marc Y Donath, Sanjoy Dtta, Joseph A. Hedrick, Stephen R. Karpen, Thomas W. H. Kay, Thomas Marder, Marjana Marinac, Jennifer McVean, Robert Meyer, Jeremy Pettus (2024). TNF-α Inhibitors for Type 1 diabetes: exploring the path to pivotal clinical trial. Frontiers in immunology 1; 15:1470677. Doi:103389/fimmu.1470677.
Boland, S. Brigid, Mamie H. Dong, Ricki Bettencourt, Elizabeth Barett-Connor, Rohit Loomba (2014): Association of serum bilirubin with aging and Mortality. Journal of Clinical and Experimental Hepatology Volume 4, Issue 1, pages 1-7.doi.org/10.1016/j.jceh.01.003.
Cakatay, U,Yanar K, Aydin S, , Mengi M, Buyukpinarbasili N, Atukeren P, (2011): Protein and DNA oxidation in different anatomic regions of rat brain in a mimetic ageing model. Journal of Basic and Clinical Pharmacology and Toxicology; 109:423-33.
Cedrone Edward, Timothy M. Potter, Barry W. Neun and Marina A. Dobrovolskaia (2020). NCL Method ITA-24 Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human TNF-α in Culture Supernatants. National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols (Internet). https://ncl.cancer.gov/resources/assay-casacade-protocols. DOI: 10.17917/JQM0-0G48.
Chattipakorn, Nipon, Cherry Bo-Htay, Siripong Palee, Nattayaporn Apaijai, Siriporn C.Chattipakorn (2018): Effects of D-galactose-induced ageing on the Heart and its Potential Interventions. Journal of Cellular and Molecular Medicine. Jan24;volume 22(3):1392-1410. Doi: 101111/jcmm.13472
Chen, M., Wang, Y., Deng, S., Lian, Z., and Yu, K. (2022). Skeletal muscle oxidative stress and inflammation in aging: Focus on antioxidant and anti-inflammatory therapy. Frontiers in Cell and Developmental Biology, 10, 964130. https://doi.org/10.3389/fcell.2022.964130
Colombini, B., Dinu, M., Murgo, E., Lotti, S., Tarquini, R., Sofi, F., and Mazzoccoli, G. (2022). Ageing and low-level chronic inflammation: The role of the biological clock. Antioxidants, 11(11), 2228. https://doi.org/10.3390/antiox11112228
Cui, X. Zuo P, Zhang Q, Li X, Hu Y, Long J, Packer L, Liu J.(2006); Chronic Systemic D-galactose exposure induceds memory loss, Neurodegeberation, and oxidative damage in mice: Protective effects of R-alpha-lipoic acid. Journal of Neuro Science Research. 2006 June; 83(8): 1584-90. Doi: 10.1002/jnr.20845. PMID:16555301.
Dare, Joseph Babatunde, Ojewale, Abdulfatai Olakunle, Olaniyan Olugbemi Tope, Adole, John Adakole, Okotie Gloria Enevwo, Akintayo, Christopher Oloruntoba, Dare, Ayobami (2021): Adansonia digitata Aqueous leaf extract ameliorates dexamethasone-induced Testicular injury in Male Wistar rats. Asian Pacific Journal of Reproduction 10(3): P 113-120.DOI: 10.4103/2305-0500.316623.
Dominiczak, M.H. (1998): Teaching and Training Laboratory Professionals for the 21st century, Clinical Chemistry and Laboratory Medicine, 36, 133-136.
Ebaid, H., Bashandy, S. A., Alhazza, I. M., Hassan, I., and Al-Tamimi, J. (2019). Efficacy of a methanolic extract of Adansonia digitata leaf in alleviating hyperglycemia, hyperlipidemia, and oxidative stress of diabetic rats. BioMed Research International, Article 2835152. https://doi.org/10.1155/2019/2835152
El-Shora, H. M., and Soror, A. S. (2021); Beneficial effect of the methanolic leaf extract of Allium hookeri on stimulating glutathione biosynthesis and preventing impaired glucose metabolism in type 2 diabetes. Archives of Biochemistry and Biophysics, 708, Article 108961. https://doi.org/10.1016/j.abb..108961.
Gama, Wiyata Arlina, Rahmawati Akib, Ummul Khaera (2025): The effect of giving D-Galactose as an Aging inducer on Body weight, Glucose levels and Interleukin-6 Levels in Wistar Rats. Journal of Community Health Provision, Volume 5, issue1, Page 001-008, DOI: http://doi.org/1055885/jchp:v5il.556.
Ghanbari-Niaki, Abbass; Saleh Rahmati-Ahmadabad; David Robert broom; Ali-Akbar Kolbadinejad; Jamal Nikbakht; Martin Hofmeister (2022): Effects of Endurance Training with or Without Rosehip Fruit (Rosa Canina) Extraction and D-galactose Solution on Plasmatic Liver Enzymes, Lipid Profiles, Selected Biochemical Variables in Male Rats. Journal of Biological and Applied Sciences. Brazilian Archieves of Biology and Technology 65. Doi.org/10.1590/1678-4324-2022210460.
Hadzi-Petrushev, N, V. Stojkovski, D. Mitrov, M. Mladenov (2015): D-galactose Induced Changes in Enzymatic Antioxidant Status in Rats of Different Ages. Physiological Research pre-press article.
Han Liu, Hongyu Zhai, Xiangyu Ma, He Li., Olaosen Ren, Jiating Liu, Zhe Zhang, Xintong Li, Qiuyue zhang, Xin Sun (2025): Exploring the Protective Effects of Gastrodia elata Extract on D-galactose-induced Liver injury in Mice based on the PI3k/Akt Signalling Pathway.Journal of Current issue in molecular biology Dec 20; 48(1):6. Doi:10.3390/cimb48010006.
Hanafy, A., Aldawsari, H. M and Abdel-Hady, S.E (2016): Evaluation of hepato[rotective activity of Adansonia digitata extract on acetaminophine-induced hepatotoxicity in rats. Journal of evidence based Complementary and Alternative Medicine, 4579149.
Helle M., Boeije L., de Groot E, de Vos A, Aarden L.(1991). Sensitive ELISA for Interleukin-6. Detection of IL-6 in Biological Fluids: Synovial fluids and Sera. Journal of Immunol Methods 8;138(1): 47-56. Doi:10.1016/0022-1759(91)90063-I.PMID:2019746.
Ho, SC, Liu JH, and Wu RY. (2003); Establishment of the mimetic aging effect in mice caused by D-galactose. Biogerontology; Vol.4(1);15-8, doi.10.1023/a:10224171102206. PMID: 12652185.
Iakovou, E., and Kourti, M. (2022). A comprehensive overview of the complex role of oxidative stress in aging and emerging antioxidant therapeutic interventions. Frontiers in Aging Neuroscience, 14, 827900. https://doi.org/10.3389/fnagi.2022.827900
Kaplan A. (1984): Bilirubin Clinical Chemistry, Published in the C.V. Mosby Co., St Louis, Toronto, pages 436, 1238-1241.
Karmen, A. (1995): Serum and plasma Aspartate Amino Transferase determination. Journal of Clinical Investigation, 24;126.
Kazeem, MI, Akanji MA, Rahman M., Hafizur, M. Choudhary (2012): Antiglycation, Antioxidant, Toxicological potential of polyphenol extracts of alligator pepper, Ginger and nutmeg from Nigeria. Asian Pacific Journal of Tropical Biomedicine Sep:Volume 2 (9): 727-732.doi 10.116/ S2221-1691 (12) 60218-4. PMID;23570003.
Kumar, Harsh, Kanchan Bhardwaj, Marian Valko, Suliman Y. Alomar, Saleh H. Alwasel, Natalia Cruz-Martins, Daljeet Singh Dhanjal, Reena Sing, Kamil Kuca, Rachna Verma, Dinesh Kumar (2022): Antioxidant potential of Lactobacillus sp. In ameliorating D-galactose-induced aging. Journal of applied microbiology and Biotechnology. 4;106 (13-16):4831-4843. Doi:10.1007/s00253-022-12041-7.
Labarrere, C. A., and Kassab, G. S. (2022). Glutathione: A life-sustaining small molecule that protects against oxidative stress, ageing and inflammation. Frontiers in Nutrition, 9, 1007816. https://doi.org/10.3389/fnut.2022.1007816
Lee, Kwon Young, Hyo Young Jung, dae Young Yoo, Woosuk kim, Jong Whi Kim, Hyun Jung Kwon, Dae Won Kim, Yeo Sung Yoon, In koo Hwang, Jung, Hoon Choi (2017): Dendropanax Morbifera Leveille extract ameliorates D-galactoise-induced memory deficits by decreasing inflammatory responses in the hippocampus. Journal of Laboratory animal Research Volume 33(4): 283-290. doi:10.5625/lar.33.4.283.
Leyane, T. S., Jeres, S. W., and Houreld, N. N. (2022). Oxidative stress in ageing and chronic degenerative pathologies: Molecular mechanisms involved in counteracting oxidative stress and chronic inflammation. International Journal of Molecular Sciences, 23(13), 7273. https://doi.org/10.3390/ijms23137273
Liu, Han, Hongyu Zhai, Xiangyu Ma. He Li, Qiasen Ren, Jiating Liu, Zhe zhang, Xintong Li, Qiuyue Zhang and Xin Sun (2026). Exploring the protective Effect of Gastrodia Elata extract on D-galactose-induced liver injury in mice based on the PI3k/Akt signaling pathway. Journal of Current Issues in Molecular Biology, 4 (1), 6. https;// doi.org/10.3390/cimb48010006.
Makena, W. U., Otong, E. S., Dibal, N. I., Bazabang, S. A., (2021). Aqueous fruit pulp extract of Adansonia digitata (L.) protects against lead-acetate-induced hepato-renal damage in rat model. Beni-Suef University Journal of Basic and Applied Sciences, 10, 59. https://doi.org/10.1186/s43088-021-00151-6.
Martemucci, G., Portincasa, P., Di Ciaula, A., Mariano, M., Centonze, V., and D’Alessandro, A. G. (2022). Oxidative stress, aging, antioxidant supplementation and their impact on human health: An overview. Mechanisms of Ageing and Development, 206, 111707. https://doi.org/10.1016/j.mad.2022.111707
Misra, H.P, and Fridovich, I. (1975). The role of Superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological chemistry. 247 (10), pp. 3170-3175
Molecular Aspects of Medicine. (2022). Ageing – Oxidative stress, post-translational modifications and disease. Molecular Aspects of Medicine, 86, 101099. https://doi.org/10.1016/j.mam.2022.101099.
Mumtaz, Shumaila, Shaukat Ali, Muhammad Zahid Qureshi, Ali Muhammad, Adul Manan, Tafail Akbar Mughal (2023): Saudi Journal of Biological Sciences, volume 30, Issue 12, 103872.
Musab, A.M., Taj Eldin, I. M., Mohammed, A. H., and Hassan, H. M. (2015); Hepatoprotective effect of Adansonia digitata (baobab) fruits pulp methanolic extract on CCl₄-induced hepatotoxicity in rats. World Journal of Pharmaceutical Research, 4(8), 368–377.(Significant reduction in total bilirubin). wjpr.s3.ap-south-1.amazonaws.com
Ohkawa, H., Ohishi, N. and Yagi, K. (1979): Assay for Lipid peroxide in animal tissues by thiobarbituric acid Reaction. Journal of Analytical biochemistry, 95 (2), pages 351-358.
Okechukwu, J. N and Ijeh, I. I. (2024); Effect of Ethanol Leaf extract of Moringa Oleifera on D-galactose induced Changes in Lipid Profile of aged Wistar rats. Journal of sustainable Veterinary and Allied Sciences, Volume 6 Issue 3: 162-166.
Oladele, Tajudeen Adekunle, Cyril-Olutayo Christiana Mojisola and Taiwo Olayemi Elufioye (2012): Ethnobotanical Survey of plants Used as memory enhancer and Antiageing in Ondo State, Nigeria. International Journal of Pharmacy. ISSN 2249-1848. Review article.
Otong, E. S., Musa, S. A., Danborno, B., and Sambo, S. J. (2021). Mitigation of lead neurotoxicity by aqueous fruit extract of Adansonia digitata in adult Wistar rats. Journal of Complementary and Alternative Medical Research, 16(4), 71–82.
Rasheed, Zafar (2024): Therapeutic potentials of Catalase: Mechanisms, applications, and future perspectives. International Journal of Health Science (Qassim) Mar-Apr; Volume 18(2); Pages 1-6. PMID: 38455600.
Reitman, S., Frankel, S. A., (1957). Colorimetric method for determination of serum glutamic-oxaloacaetic and glutamic-pyruvic transaminases. American Journal of Clinical Pathology 28, 56-62.
Rincon, Mercedes (2012): Interleukin-6: from an inflammatory marker to a target for inflammatory diseases. Journal of Trends in Immunology; Volume 33, Issue 11, pages 571-577
Rita, K., Bernardo, M. A., Silva, M. L., Brito, J., Mesquita, M. F., Pintão, A. M., and Moncada, M. (2022). Adansonia digitata L. (baobab fruit) effect on postprandial glycemia in healthy adults: A randomized controlled trial. Nutrients, 14(2), 398. https://doi.org/10.3390/nu14020398.
Rufai, M.S, Shamsudden u, and Usman A.D (2006): Toxicity Studies of Ethanol Leaf Extract of Adansonia digitata. Bayero Journal of Pure and Applied Sciences, Volume 13(1): 478-484ISSN -6996.
Sa’id, A. M., Musa, A. H., Mashi, J. A., Maigari, F. U., and Nuhu, M. N.(2020); Phytochemical screening and hepatoprotective potential of aqueous fruit pulp extract of Adansonia digitata against CCl₄-induced liver damage in rats. Asian Journal of Biochemistry, Genetics and Molecular Biology, 3(3), 12–21. (Dose-dependent reduction in bilirubin).
Saleh, O. Dalia, Dina F. Mansour, Ingy M. Hashad, Rofanda M. Bakeer (2019): Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of Keap-1/nrf-2 parthway. Journal of Pharmacology. Volume 855, pages 40-49.
Shati, A. A., Elsaid, F. G., El-Kott, A. F., and Amin, A. H. (2016); Effect of Adansonia digitata on indole-3-acet acid-induced hepato-nephrotoxicity in rats. Basic and Applied Sciences Journal, 2(1). (Reported hepatoprotective effects with changes in total bilirubin). journals.kku.edu.sa
Shi, Shaoqi, Xiaoxia Li, Feng Zhang, Zhengqiang Jiang, Jing Wang, Liang Zhao, Juan Chen , Xi Shu, Bing Fang, Ping Liu, Jingjing He, Shaoyang Ge, Fuqing Wang, Jie Guo, Yixuan Li, Jie Luo, Ran Wang (2024): Alleviating D-Galactose-Induced Aging in Mice by Modulating Gut-Liver Axis Using Lactiplantibacillus plantarum TY-Y10. Foods. Nov.13;Volume 13(22): 3618.doi:10.3390/foods13223618.
Shwe, Thazin, Wasana Pratchayasakul, Nipon Chattipakorn, Siriporn C. Chattipakorn (2018): Role of D-galactose-induced brain aging and its potential used for therapeutic interventions. Journal of Experimental Gerontology, Volume 101, pages 13-36.
Silva, M. L., Rita, K., Bernardo, M. A., Mesquita, M. F., Pintão, A. M., and Moncada, M. (2023). Adansonia digitata L. (baobab) bioactive compounds, biological activities, and the potential effect on glycemia: A narrative review. Nutrients, 15(9), 2170. https://doi.org/10.3390/nu15092170
Songkiatisak, P., Rahman, S. M. T., Aqdas, M., and Sung, M. H. (2022). NF-κB, a culprit of both inflamm-ageing and declining immunity? Immunity & Ageing, 19, 20. https://doi.org/10.1186/s12979-022-00277-w
Szasz, G., (1969): A kinetic photometry method for Serum gamma-glutamyl transpeptidase. Journal of Clinical Chemistry 15: 124-136.
Tartiere Antonio Garcia-Bernado, Jose MP Freije, Carlos Lopez-Otin (2024). The hallmarks of aging as a conceptual framework for health and longevity research. Frontiers in Aging. DOI: 10.3389/fragi.1334261.
Tayyaba, Afsar, Suhail Razak, Muhammad Rashid Khan, Saadia Mawash, Ali Almajwal, Maria Shabir and Ihsan UI Haq (2016): Evaluation of antioxidant. Anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts. BMC complementary and Alternative Medicine 16:258 DOI 10.1186/s 12906-016-1240-8.
Uhuo, E. N., Egba, S. I., Nwuke, P. C., Obike, C. A., and Kelechi, G. K. (2022). Antioxidative properties of Adansonia digitata L. (baobab) leaf extract exert protective effect on doxorubicin-induced cardiac toxicity in Wistar rats. Journal of Clinical Nutrition Open Science, 45, 3–16. https://doi.org/10.1016/j.nutos.2022.07.004
Vaillant, Justiz A. Angel and Ahmad Qurie (2022): Interleukin; Startpearls (internet). National Centre for Biotechnology information.
Waggiallah, Hisham and Mohammed Alzohairy (2011): The effect of Oxidative stress on human red cells glutathione reductase level, and prevalence of anemia among diabetics. North American Journal of Medical Sciences, doi: 10.4297/najms.3344.
Wei, J. Tan, Z., Huang, G., Zeng, Y., Chen, S., Yuan, G., He, S. He, S. and Zhou, Y. (2025): Anthocyanins delay D-galactose-induced mouse liver ageing by regulating the NF-kB/IKK signaling pathway. Journal of Food Science and Nutrition, 13 (4), e70161.
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Copyright (c) 2026 Emmanuel Olagoke, Mrs Babalola Omolara, Professor O.T Adedosu, Professor G.E. Adeleke, Mrs Masaudat Adebayo, Mr. Abdulrasak Mohammed , Mr. Adetayo Akinboro, Mrs Olagoke Oluwatosin
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