Journal of Chemistry and Nutritional Biochemistry https://sabapub.com/index.php/jcnb <p>Journal of Chemistry and Nutritional Biochemistry(JCNB) is a peer reviewed international journal published by Saba Publishing. The aim of the journal is to provide a venue for researchers and practitioners to share theories, views, research results and classroom practices in areas of Chemistry , Nutritional , Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Articles are published in English.</p> <p><strong>Editor in Chief: <a href="https://www.scopus.com/authid/detail.uri?authorId=56719770900" target="_blank" rel="noopener">Dr. Ammar Mohammed Hamood AL-Farga</a></strong><br /><strong>ISSN (online)</strong>:<a href="https://portal.issn.org/resource/ISSN/2709-5932" target="_blank" rel="noopener"> 2709-5932</a><br /><strong>Frequency:</strong> Semiannual</p> SABA Publishing en-US Journal of Chemistry and Nutritional Biochemistry 2709-5932 Anti-hyperglyceamic Effects of Psidium guajava LINN Crude Leaf Extracts and Fractions in Alloxan-induced Diabetic Mice https://sabapub.com/index.php/jcnb/article/view/283 <p>In Nigeria, rural inhabitants often resort to herbal remedies and dietary control for the treatment and management of various forms of diabetes mellitus. This study was conducted to provide the rationale for the use of Psidium guajava leaves as a potent traditional anti-diabetic remedy. The crude leaf extracts of n-hexane, methanol, and ethyl acetate of Psidium guajava were separately prepared by cold maceration. Then, ethyl acetate crude extract of Psidium guajava leaves was fractionated by column chromatography to yield ethyl acetate bulked fractions: EF-I (1-75), EF-II (76-150), and EF-III (151-250). The n-hexane, ethyl acetate, and methanol crude leaf extracts and ethyl acetate bulked fractions (EF-I, EF-II and EF-III) were evaluated for anti-diabetic activity in alloxan-induced diabetic mice. The blood sugar levels of treated and untreated alloxan-induced diabetic mice were assayed as indices of anti-diabetic effect. The phytochemical constituents of both crude extracts and ethyl acetate fractions of Psidium guajava leaves and the mean lethal dose (LD<sub>50</sub>) of ethyl acetate crude leaf extract of Psidium guajava were determined. The mean lethal dose (LD<sub>50</sub>) of ethyl acetate crude leaf extract was calculated to be 1500mg/kg b.w. The results indicated that oral administration of ethyl acetate, n-hexane, methanol crude extracts, and ethyl acetate bulked fractions of Psidium guajava leaves at a dose of 100mg/kg b.w on treated groups exhibited much significant[p&lt;0.001, p&lt;0.01and p&lt;0.05]anti-hyperglyceamic effect by ameliorating high blood sugar levels of alloxan-induced diabetic treated mice, while EF-II and EF-III showed non-significant[p&gt;0.05] anti-hyperglyceamic activity for the reduction in blood sugar levels compared with the negative and positive control groups. The anti-diabetic potency of the crude leaf extracts and ethyl acetate fractions were in the order; EC&gt;HC&gt;MC&gt;EF-I&gt;EF-II&gt;EF-III. The results of phytochemical screening of the crude extracts and ethyl acetate bulked fractions showed the presence of tannins, flavonoids, saponins, alkaloids, terpenoids, glycosides, and steroids while reducing sugar was absent. The results from this study gives credence to the use of Psidium guajava as an anti-diabetic agent in the management of diabetes mellitus.</p> Uchenna Nwabunwanne Eze Ugwu Chukwuebuka V Onuoha Maxwell Ubenyi Stanley M Copyright (c) 2021 Journal of Chemistry and Nutritional Biochemistry https://creativecommons.org/licenses/by/4.0 2021-08-31 2021-08-31 2 2 1 27 10.48185/jcnb.v2i2.283 Experimental Analysis Of Generic Antibiotics Along With Honey Sensitivity Against Microbial Flora Obtained From Frozen Hilsa Fish (Tenualosa ilisha) https://sabapub.com/index.php/jcnb/article/view/230 <p>This study deals with the in-vitro experimental analysis of different generic antibiotics and season based honey, sensitivity against isolated bacteria. The characterization of bacteria isolated from the frozen hilsa is done through various biochemical reactions The antibacterial sensitivity of honey and antibiotics are evaluated through zone of inhibition analysis of bacterial colony. So, it is obvious that this experiment provides providential values for future research including food value analysis.</p> Sunandan Dey Copyright (c) 2021 Journal of Chemistry and Nutritional Biochemistry https://creativecommons.org/licenses/by/4.0 2021-08-31 2021-08-31 2 2 28 34 10.48185/jcnb.v2i2.230